Press Release 054/2023

Materials Research: Biocatalytic Foams of Tremendous Stability and Activity

KIT Researchers Use Enzymes to Develop a New Class of Materials for Biocatalytic Processes
2023_054_Materialforschung_Biokatalytische Schaeume mit enormer Haltbarkeit und Aktivitaet_72dpi
New biomaterials for industrial biocatalysis: Enzyme foams form three-dimensional porous networks with a stable hexagonal honeycomb structure. (Photo: Julian Hertel, KIT)

Industrial biocatalysis with enzymes is deemed to be a “game changer” in the development of a sustainable chemical industry. Enzymes can be used to synthesize an impressive range of complex molecules, including pharmaceutical substances, under environmentally compatible conditions. Researchers of Karlsruhe Institute of Technology (KIT) have now developed a new class of materials by producing enzyme foams of tremendous stability and activity. They report in Advanced Materials. The researchers have already filed a patent application on the process to produce enzyme foams. (DOI: 10.1002/adma.202303952)

Industrial biocatalysis is mainly used for the production of pharmaceuticals and special chemicals. To advance this process, researchers are working on new process technologies. In biocatalysis, enzymes accelerate the reactions instead of chemical catalysts. This saves resources and energy. Current work is aimed at continuously providing large quantities of enzyme biocatalysts under conditions that are as gentle as possible. To achieve efficient substance conversions, enzymes are immobilized in microstructured flow reactors. They are spatially fixed and bound to an inert material. Hence, their mobility is limited, which results in a higher concentration of enzymes and a higher productivity.

Foamed Microdroplets from Self-assembling Enzymes

Normally, foaming modifies the enzyme structure and enzymes lose their biocatalytic activity. The new protein foams, however, have a tremendous stability and activity. Activity is a measure of the effectiveness of the enzyme that ensures that the initial substances react with each other as quickly as possible. For the production of protein foams, two dehydrogenase enzymes are mixed, which carry matching sites to spontaneously form a stable protein network. “Then, a gas flow is added to this mix in a microfluidic chip for the controlled formation of microscopic bubbles of uniform size,” explains Professor Christof Niemeyer from the Institute for Biological Interfaces 1. The foam with the uniform bubble size is applied directly onto plastic chips and dried, as a result of which the proteins polymerize and form a stable hexagonal lattice.

“These monodisperse all-enzyme foams are three-dimensional porous networks consisting of biocatalytically active proteins exclusively,” Niemeyer says. The stable hexagonal honeycomb structure of the foams has a mean pore diameter of 160 µm and a thickness of the lamellae of 8 µm. It is formed by the uniform spherical bubbles after a few minutes.

Efficient Use of the Active and Stable Full-enzyme Foams

To efficiently use the enzymes for conversion reactions, they have to be immobilized in large quantities under most gentle conditions in order to maintain their activity. So far, enzymes have been immobilized on polymers or carrier particles. However, these require valuable reactor space and enzyme activity may be reduced. “Compared to our ‘all-enzyme hydrogels,’ the new foam-based materials have a far larger surface area on which the desired reaction can take place,” Niemeyer points out. In contrast to theoretically expected results, the new foams surprisingly show strikingly high durability, mechanical resistance, and catalytic activity of the enzymes, which had not previously been achieved in foaming proteins. The researchers assume that stability results from the matching junctions of the enzymes. They enable the enzymes to self-assemble and to form a new material network of unmatched stability during drying. “Surprisingly, the newly developed enzyme foams are far more stable after drying for four weeks than the same enzymes without foams,” Niemeyer says. “This is particularly useful for the commercialization of the process, as production on stock and delivery are facilitated considerably.”

The new biomaterials open up many opportunities for innovations in industrial bioengineering, materials sciences, or food technology. Protein foams might be used in biotechnological processes to produce valuable compounds more efficiently and sustainably. The researchers, for instance, used the foams to produce tagatose which is a promising alternative to refined sugar.

Original Publication
Julian S. Hertel, Patrick Bitterwolf, Sandra Kröll, Astrid Winterhalter, Annika J. Weber, Maximilian Grösche, Laurenz B. Walkowsky, Stefan Heißler, Matthias Schwotzer, Christof Wöll, Thomas van de Kamp, Marcus Zuber, Tilo Baumbach, Kersten S. Rabe, Christof M. Niemeyer: Biocatalytic Foams from Microdroplet-Formulated Self-Assembling Enzymes. Advanced Materials, 2023. DOI: 10.1002/adma.202303952

More about the KIT Center Materials in Technical and Life Sciences


Being “The Research University in the Helmholtz Association”, KIT creates and imparts knowledge for the society and the environment. It is the objective to make significant contributions to the global challenges in the fields of energy, mobility, and information. For this, about 10,000 employees cooperate in a broad range of disciplines in natural sciences, engineering sciences, economics, and the humanities and social sciences. KIT prepares its 22,800 students for responsible tasks in society, industry, and science by offering research-based study programs. Innovation efforts at KIT build a bridge between important scientific findings and their application for the benefit of society, economic prosperity, and the preservation of our natural basis of life. KIT is one of the German universities of excellence.

sfo, 27.07.2023

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