Miniaturizing the Test Tube with Lipid Nanotechnology
Institute of Nanotechnology
Bldg. 640 Seminar room 0-167
Talk given by
Prof. Steven Lenhert
Department of Biological Science
Florida State University,
Miniaturization of laboratory procedures is opening new possibilities in medicine by allowing point of care diagnostics, precision medicine, novel therapeutics, reliable manufacturing of biologics, as well as applications that are likely unpredictable a priori. A fundamental challenge, however, is in the miniaturization of the test-tube. As compartments get smaller, surface effects begin to dominate over gravity and handling of fluids requires new strategies that take advantage of nanoscale effects. Biological systems provide a solution to this problem through the formation of fluid cellular and sub-cellular compartments defined by lipid bilayers as the boundaries. Inspired by this approach, Lenhert’s research in the last five years has focused on the formation of arrays of lipid droplets on surfaces in order to effectively miniaturize the test tube for high throughput screening of small molecule drugs and as nanostructured biosensor elements. Recent progress in the following areas will be presented: (1) lipid multilayer nanofabrication on surfaces, (2) drug screening microarrays for cell culture, and (3) the use of lipid nanostructures as sensor arrays and model membrane systems for testing hypotheses about how cells organize their molecules.
1. T. W. Lowry, A. Kusi-Appiah, J. J. Guan, D. H. Van Winkle, M. W. Davidson, S. Lenhert, (2014) Materials Integration by Nanointaglio. Advanced Materials Interfaces 1, 1300127.
2. A. E. Kusi-Appiah, T. W. Lowry, E. M. Darrow, K. A. Wilson, B. P. Chadwick, M. W. Davidson, S. Lenhert, (2015) Quantitative Dose-Response Curves from Subcellular Lipid Multilayer Microarrays. Lab Chip 15, 3397-3404.
3. T. W. Lowry, H. Hariri, P. Prommapan, A. Kusi-Appiah, N. Vafai, E. A. Bienkiewicz, D. H. Van Winkle, S. M. Stagg, S. Lenhert, (2016) Quantification of Protein-Induced Membrane Remodeling Kinetics In Vitro with Lipid Multilayer Gratings. Small 12, 506-515.
Prof. Steven Lenhert
Florida State University, Tallahassee, USA
Dr. Dr. Michael Hirtz
Institute of Nanotechnology (INT)
Karlsruhe Institute of Technology (KIT)
E-Mail:michael hirtz∂kit edu
Interessierte / Jede(r)