Press Release 129/2016

Scavenger Cells Repair Muscle Fibers

New Findings Give Insight into the Cell Membrane Repair Process of Torn Muscle Fibers
Scavenger cell removes the repair patch. Scale bar: 4 µm. (Photo: Volker Middel / KIT)
Scavenger cell removes the repair patch. Scale bar: 4 µm. (Photo: Volker Middel / KIT)

Everybody knows the burning sensation in the legs when climbing down a steep slope for a long time. It is caused by microruptures in the cell membrane of our muscle fibers. These holes in the cell envelopes must be closed as soon as possible as otherwise muscle cells will die off. Researchers at KIT were able to observe this repair process using high-resolution real-time microscopy. It only takes a few seconds until proteins from the inside of the injured cell form a repair patch that finally closes the hole in the membrane. The researchers at KIT now demonstrated that scavenger cells moving around within the muscle virtually perform nano-surgery to remove this repair patch later and restore the normal cell membrane structure. (DOI: 10.1038/NCOMMS12875)  

Video:

www.nature.com/articles/ncomms12875#supplementary-information


The cells of our skeletal muscles have effective mechanisms for the repair of ruptures in their cell membranes. These ruptures are due to mechanical stress to which we expose our muscles even when doing healthy exercises. The cell membrane is an important barrier which is essential to the proper functioning and survival of cells. If this barrier collapses and cannot be repaired quickly, the muscle cell will die, resulting in a loss of muscle mass. People whose repair proteins, e.g. dysferlin, do not work properly develop atrophy of the muscles which leads to most severe disabilities and premature death. 

 

In an interdisciplinary cooperation project of the KIT research teams led by Uwe Strähle and Gerd Ulrich Nienhaus, the PhD students Volker Middel and Lu Zhuo developed new techniques to observe membrane repair processes with ultra-high resolution in real time in human cells and in muscle cells of zebrafish embryos. They proved that the repair patch assembling itself from repair proteins, such as dysferlin or annexines, also accumulated the phosphatidylserine lipid. Phosphatidylserine is a known appetizer for scavenger cells, the so-called macrophages.

 

The KIT researchers presented a movie that shows how the macrophages indeed latch to the repair patch and eat it up. Only after the patch has been removed, the cell envelope is fully restored.  Thus, the repair of the membrane in muscle fibers requires, in addition to the formation of repair patches in the injured cell, the aid of macrophages roaming around within the muscle. The researchers further demonstrated that a short amino acid sequence in the dysferlin repair protein is responsible for the phosphatidylserine transport. It is remarkable that there are myopathy patients who have a defect precisely in this sequence of the dysferlin protein. The new findings may therefore contribute to the development of therapies against muscle atrophy.

Video:

www.nature.com/articles/ncomms12875#supplementary-information

 

Being “The Research University in the Helmholtz Association”, KIT creates and imparts knowledge for the society and the environment. It is the objective to make significant contributions to the global challenges in the fields of energy, mobility, and information. For this, about 9,800 employees cooperate in a broad range of disciplines in natural sciences, engineering sciences, economics, and the humanities and social sciences. KIT prepares its 22,300 students for responsible tasks in society, industry, and science by offering research-based study programs. Innovation efforts at KIT build a bridge between important scientific findings and their application for the benefit of society, economic prosperity, and the preservation of our natural basis of life. KIT is one of the German universities of excellence.

lg, 19.09.2016
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